human monocyte nucleofector kit (Lonza)
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Lonza
human monocyte nucleofector kit
Human Monocyte Nucleofector Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+monocyte+nucleofector+kit/4d+nucleofector/us12319930-609-19-24
Average 90 stars, based on 1 article reviews
Human Monocyte Nucleofector Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+monocyte+nucleofector+kit/4d+nucleofector/us12319930-609-19-24
Average 90 stars, based on 1 article reviews
human monocyte nucleofector kit - by Bioz Stars,
2026-09
90/100 stars
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Transfection:Article Title: The Protein Kinase aPKC as Well as the Small GTPases RhoA and Cdc42 Regulates Neutrophil Chemotaxis Partly by Recruiting the ROCK Kinase to the Leading Edge Article Snippet: Stealth RNAi siRNA Negative Control Med GC Duplex #2 (Thermo Fisher Scientific) was used as a negative control. .. For siRNA transfection, PLB‐985 cells were differentiated for 3 days with the RPMI medium containing 1.25% DMSO and then nucleofection was performed using Article Title: The Protein Kinase aPKC as Well as the Small GTPases RhoA and Cdc42 Regulates Neutrophil Chemotaxis Partly by Recruiting the ROCK Kinase to the Leading Edge Article Snippet: .. For preparation of FLAG–aPKCι‐expressing PLB‐985 cells, transfection with its encoding plasmid was performed using the Article Title: Caspase Inhibition Modulates Monocyte-Derived Macrophage Polarization in Damaged Tissues. Article Snippet: Mitochondrial mass, mitochondrial superoxides, intracellular calcium and cytosolic ROS were measured by incubating the cells with 100 nM MitoTracker green (30 min at 37 ◦C) or 2.5 μM MitoSOX Red Mitochondrial Superoxide Indicator (10 min at 37 ◦C) or 2 μM Fluo-3-AM for (30 min at 37 ◦C) or 0.5 μM H2DCFDA (30 min at 37 ◦C) (all by Thermo Fisher Scientific), respectively, before flow analysis of fluorescence intensity. .. When indicated, monocytes were transfected with Article Title: Bioinformatics analysis of PLA2G7 as an immune-related biomarker in COPD by promoting expansion and suppressive functions of MDSCs. Article Snippet: Background: Immune mechanism is involved in the pathogenesis of chronic obstructive pulmonary disease (COPD).. However, the exact immune pathogenesis still remains unclear.. This study aimed to identify the immune-related biomarkers in COPD through bioinformatics analysis and its potential molecular mechanism. Recombinant:Article Title: ARL8B mediates lipid droplet contact and delivery to lysosomes for lipid remobilization. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti LAMP1 (1:500) DSHB Cat# H4A3 [RRID: AB_2296838] Rabbit polyclonal anti LAMP2A (1:250) Thermo Cat # 51–2200 [RRID: AB_87643] Mouse monoclonal anti FLAG (1:500) Sigma Cat# F1804 [RRID: AB_262044] Rabbit polyclonal anti tRFP (1:500) Evrogen Cat# AB233 [RRID: AB_2571743] Goat polyclonal anti mouse IgG, Alexa Fluor 633 (1:1000) Thermo Fisher Cat# A21052 [RRID: AB_2535719] Goat polyclonal anti Rabbit IgG, Alexa Fluor 633 (1:1000) Thermo Fisher Cat# A21071 [RRID: AB_2535732] Rabbit polyclonal anti-human TIP47 IgG Novus Cat#NB110-40764 [RRID: AB_715116] Rabbit Polyclonal Anti ARL8B(1:1000) Proteintech Cat# 13049-1-AP [RRID:AB_2059000] Rabbit Polyclonal Anti mCherry(1:1000) Abcam Cat# ab167453 [RRID:AB_2571870] Rabbit polyclonal Anti PLIN2 (1:1000) Abcam Cat# ab108323 [RRID:AB_10863476] Mouse monoclonal to GAPDH (1:10,000) Abcam Cat# ab8245 [RRID:AB_2107448] Mouse monoclonal to b-actin (1:1000) Santa Cruz biotechnology Cat#sc-47778 [RRID:AB_2714189] DyLight 800 Sheep Anti-Rabbit IgG Bio-rad Cat# STAR36D800GA [RRID:AB_10843432] IRDye Goat Anti Rabbit IgG 800CW (1:15,000) LI-COR Cat# 926–32211 [RRID:AB_621843] IRDye Goat Anti Mouse IgG 680LT (1:20,000) LI-COR Cat# 926–68020 [RRID:AB_10706161] Chemicals, peptides, and recombinant proteins Lipofectamine LTX Thermo Fisher Cat # 15338100 Dharmafect-1 Thermo Fisher Cat# T-2001-03 Dharmafect-3 Thermo Fisher Cat# T-2003-03 Lipofectamine 3000 Thermo Fisher Cat #L3000015 High Performance Liquid Chromatography:Article Title: ARL8B mediates lipid droplet contact and delivery to lysosomes for lipid remobilization. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti LAMP1 (1:500) DSHB Cat# H4A3 [RRID: AB_2296838] Rabbit polyclonal anti LAMP2A (1:250) Thermo Cat # 51–2200 [RRID: AB_87643] Mouse monoclonal anti FLAG (1:500) Sigma Cat# F1804 [RRID: AB_262044] Rabbit polyclonal anti tRFP (1:500) Evrogen Cat# AB233 [RRID: AB_2571743] Goat polyclonal anti mouse IgG, Alexa Fluor 633 (1:1000) Thermo Fisher Cat# A21052 [RRID: AB_2535719] Goat polyclonal anti Rabbit IgG, Alexa Fluor 633 (1:1000) Thermo Fisher Cat# A21071 [RRID: AB_2535732] Rabbit polyclonal anti-human TIP47 IgG Novus Cat#NB110-40764 [RRID: AB_715116] Rabbit Polyclonal Anti ARL8B(1:1000) Proteintech Cat# 13049-1-AP [RRID:AB_2059000] Rabbit Polyclonal Anti mCherry(1:1000) Abcam Cat# ab167453 [RRID:AB_2571870] Rabbit polyclonal Anti PLIN2 (1:1000) Abcam Cat# ab108323 [RRID:AB_10863476] Mouse monoclonal to GAPDH (1:10,000) Abcam Cat# ab8245 [RRID:AB_2107448] Mouse monoclonal to b-actin (1:1000) Santa Cruz biotechnology Cat#sc-47778 [RRID:AB_2714189] DyLight 800 Sheep Anti-Rabbit IgG Bio-rad Cat# STAR36D800GA [RRID:AB_10843432] IRDye Goat Anti Rabbit IgG 800CW (1:15,000) LI-COR Cat# 926–32211 [RRID:AB_621843] IRDye Goat Anti Mouse IgG 680LT (1:20,000) LI-COR Cat# 926–68020 [RRID:AB_10706161] Chemicals, peptides, and recombinant proteins Lipofectamine LTX Thermo Fisher Cat # 15338100 Dharmafect-1 Thermo Fisher Cat# T-2001-03 Dharmafect-3 Thermo Fisher Cat# T-2003-03 Lipofectamine 3000 Thermo Fisher Cat #L3000015 Plasmid Preparation:Article Title: The Protein Kinase aPKC as Well as the Small GTPases RhoA and Cdc42 Regulates Neutrophil Chemotaxis Partly by Recruiting the ROCK Kinase to the Leading Edge Article Snippet: .. For preparation of FLAG–aPKCι‐expressing PLB‐985 cells, transfection with its encoding plasmid was performed using the Gene Expression:Article Title: Mitochondrial fatty acid oxidation regulates monocytic type I interferon signaling via histone acetylation Article Snippet: HDAC inhibitor TSA (Cayman) was used in cell culture at 50 μM for 16 hours before subsequent assays. .. ON-TARGETplus siRNA for knocking down gene expression of ACAT1 , ACLY , CRAT , CACT , or nontargeting control siRNA was purchased from Horizon Discovery. siRNA against human genes or control siRNA was incubated with a mixture of a nucleofection solution ( Control:Article Title: Mitochondrial fatty acid oxidation regulates monocytic type I interferon signaling via histone acetylation Article Snippet: HDAC inhibitor TSA (Cayman) was used in cell culture at 50 μM for 16 hours before subsequent assays. .. ON-TARGETplus siRNA for knocking down gene expression of ACAT1 , ACLY , CRAT , CACT , or nontargeting control siRNA was purchased from Horizon Discovery. siRNA against human genes or control siRNA was incubated with a mixture of a nucleofection solution ( Incubation:Article Title: Mitochondrial fatty acid oxidation regulates monocytic type I interferon signaling via histone acetylation Article Snippet: HDAC inhibitor TSA (Cayman) was used in cell culture at 50 μM for 16 hours before subsequent assays. .. ON-TARGETplus siRNA for knocking down gene expression of ACAT1 , ACLY , CRAT , CACT , or nontargeting control siRNA was purchased from Horizon Discovery. siRNA against human genes or control siRNA was incubated with a mixture of a nucleofection solution ( Electroporation:Article Title: Cell specific transcriptional regulatory sequences and uses thereof Article Snippet: .. Peripheral blood mononuclear cells (PBMC) (obtained from healthy controls with written consent, (REB #3527)) were nucleofected by electroporation using Expressing:Article Title: Cell specific transcriptional regulatory sequences and uses thereof Article Snippet: .. Peripheral blood mononuclear cells (PBMC) (obtained from healthy controls with written consent, (REB #3527)) were nucleofected by electroporation using Flow Cytometry:Article Title: Cell specific transcriptional regulatory sequences and uses thereof Article Snippet: .. Peripheral blood mononuclear cells (PBMC) (obtained from healthy controls with written consent, (REB #3527)) were nucleofected by electroporation using |